The Anti-D shot is only effective within 72 hours of maternal-fetal blood mixing. So even if the injection is given at 28 weeks, it will only help if maternal and fetal blood has mixed 72 hours or less before the injection. The Rhogam manufacturer states that an injection every 12 weeks will maintain a "passive immunity," meaning that theoretically a shot every twelve weeks will protect you in the event of trauma causing your and your baby's blood to mix. They still recommend a new shot at the time of blood mixing if >15mL of exposure has occurred.
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