Tess, I also use the Lullaby Conceptions ones, the instructions are "Read results between 5 to 10 mins. Positive results may appear as early as one minute depending on the concentration of LH in urine sample but wait for 10 mins to confirm results."

It also says that a true positive is when the test line is as dark as, or darker than, the control line. Therefore, a line lighter than the control line is neg. Hopefully Jodie (from Lullaby Conceptions) is floating around and can help you out with this one. Shes' a great help!