Hi there

Its best to not do an OPK with FMU (first morning urine) as you could get a false positive with an OPK because your urine is more concentrated. So that is probably why your 11am one came negative.

Would be best to do another OPK this afternoon, just to be use. When I am using OPKs I do 2 a day - 1 at around 10am and another about 2/3pm so ensure I catch the surge.

Also, if this mornings was your +OPK the general rule is that your egg will be released up to 48hrs after the positive OPK, so I dont think you have missed your chance at all.

Good luck!