Runnermum, I second the others. I wouldn't read too much into a change in the last two cycles, even though you have been regularly having 27 day cycles up till this point. It is quite normal for an illness or accident to delay a period. Any kind of shock to the body can do it (even episodes of emotional stress).

You seem pretty convinced that you are not pregnant but I would take a test anyway. You can never be sure.

We are about the same age so I just wanted you to know that it is also very normal to take longer to conceive when you're in your late 30s. That doesn't mean it's any less frustrating (as I well know). You've been trying for number 2 for 12 months now so have you perhaps considered seeing a fertility specialist? If you aren't pg this time and are still worried about your cycles, it is probably worth getting a referral.

Tell me to mind my own business if you like ... =)