I lay in bed for 1.5 hours this morning before testing coz I woke up at 4am! I was busting but I didn't want to wake up dh too early! I think it was worth the wait though.......if I were you I would test at 4am, as Jasper said, if that's your longest period of sleep. Make sure you wake up properly though so you don't miss the stick LOL! Good luck