When DD was a little baby, it was the ants come marching, then it was twinkle, twinkle - all 3 verses, and that still helps calm her down. DS so far prefers for me to la,la rather than actually sing and that tends to be any tune I can think of!
For DS1 it has always been "hush little baby", he was really sick for 12months & I would sing it all the time....he's now 3 and I've caught him singing it to DS2 (so cute).
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