Hi girls, can anyone advise me on if they had trouble on Puregon with E2 (estrogen) levels being too low at first.. Did they rise, what dosage were you given. ?? Have had 2nd blood test and E2 is still on 36 which is very low so I am now up to 550 Puregon with a little shot of Pregnyl (little trigger) every 3rd day... Next blood test this thursday and I am worried... thankyou for reading and responding if possible...